利迪链霉菌M01对番茄生长、青枯病发病率及根际细菌群落组成的影响

Effects of Streptomyces lydicus M01 on growth, bacterial wilt incidence, and rhizosphere bacterial community composition of tomatoes

摘要:

【背景】利迪链霉菌(Streptomyces lydicus)对多种作物均有较好的促生效果，且对病原真菌具有广谱抑制作用，但该菌对细菌性青枯病的防控研究较少。【目的】探究利迪链霉菌M01能否促进番茄生长并抑制番茄青枯病，以及M01对番茄生长的影响是否通过影响根际细菌群落结构实现。【方法】采用温室盆栽试验和扩增子高通量测序技术研究M01对番茄生长、青枯病发病率及根际细菌群落组成的影响。【结果】施用利迪链霉菌M01的番茄植株鲜重、干重、株高、用土壤与作物分析开发(soil and plant analyzer develotrnent, SPAD)方法测量的叶绿素浓度、根系活力和植株P含量比对照分别提高了22.7%、12.5%、16.0%、28.1%、18.4%和17.9%，其中对株高、SPAD值和植株磷含量影响显著(P<0.05)。M01处理延缓了番茄青枯病的发病时间，接种9周后发病率比对照降低了41.8%。此外，M01对番茄根际细菌群落无显著影响(门水平群落组成，P=0.4；属水平群落组成，P=0.4)。【结论】利迪链霉菌M01可促进番茄植株生长并抑制番茄青枯病，利迪链霉菌M01对番茄生长的影响并非通过调控根际细菌群落实现。

Abstract:

[Background] Streptomyces lydicus can efficiently promote the growth of many crops and inhibit many pathogenic fungi. However, there are few studies on the biocontrol effects of S. lydicus on bacterial wilt disease. [Objective] To explore whether S. lydicus M01 can promote tomato growth and inhibit tomato bacterial wilt disease and whether the effect of S. lydicus M01 on tomato growth is achieved by affecting the bacterial community structure in the rhizosphere.[Methods] The effects of S. lydicus M01 on plant growth, bacterial wilt incidence, and rhizosphere bacterial community composition of tomatoes were explored by the greenhouse pot experiments and high-throughput amplicon sequencing. [Results] Compared with the control, S. lydicus M01 increased fresh weight, dry weight, plant height, chlorophyll concentrations measured using soil and plant analyzer develotrnent methods, root vigor, and P content of tomato plants by 22.7%, 12.5%, 16.0%, 28.1%, 18.4%, and 17.9%, respectively. S. lydicus M01 significantly increased plant height, SPAD value, and P content of tomato plants (P<0.05). S. lydicus M01 delayed the onset time of bacterial wilt and decreased bacterial wilt incidence by 41.8% at 9 weeks after pathogen inoculation. Additionally, S. lydicus M01 had no significant effects on rhizosphere bacterial community composition (P=0.4 for microbiome composition at the phylum level and P=0.4 for microbiome composition at the genus level). [Conclusion] S. lydicus M01 can promote plant growth of tomatoes and suppress bacterial wilt of tomatoes, and these effects are not achieved by regulating rhizosphere bacterial community composition.