灵芝尿嘧啶营养缺陷型菌株的筛选和分子鉴定

Screening and molecular verification of uracil auxotrophic mutants of Ganoderma lucidum

摘要:

【背景】营养缺陷型是一种应用广泛的分子标记，但是目前在灵芝中还未有研究和应用报道。【目的】为灵芝遗传转化研究、杂交育种和菌种鉴别提供亲本材料和技术支持。【方法】采用紫外光诱变、单单杂交、孢子单核化的方法从灵芝单核体菌株出发得到尿嘧啶营养缺陷型双核体菌株。【结果】获得8株稳定的尿嘧啶营养缺陷型单核体突变菌株和7株尿嘧啶营养缺陷型双核体菌株。 【结论】灵芝尿嘧啶营养缺陷型菌株在添加外源营养物的基础上可恢复正常生长，可以为灵芝遗传转化体系的构建和灵芝育种提供材料。

关键词:灵芝，5-氟乳清酸，尿嘧啶营养缺陷型，双核体

Abstract:

[Background] Auxotrophy is a widely applied biomarker in production and scientific research, but there is no report on Ganoderma lucidum. [Objective] This study was conducted to provide parent strains and technique supports for genetics research, cross breeding and spawn identification of Ganoderma lucidum. [Methods] The uracil auxotrophic mutant strains were obtained by UV mutagenesis of protoplast. The randomly selected uracil auxotrophic monokaryon strains were used as parents, and crossed with each other by Monkaryon-monkaryon mating to establish uracil auxotrophic dikaryons. [Results] eight uracil auxotrophic mutant strains were obtained by UV mutagenesis of protoplast. seven uracil auxotrophic dikaryon strains were obtained by Monkaryon-monkaryon crossing. [Conclusion] The testing results showed these auxotrophic strains restored their growth on PDA medium with uracil. The uracil auxotrophic dikaryon strains would be used as a tool for genetic transformation study and breeding.

Key words:Ganoderma lucidum, 5′-Fluoro-oritic acid, Uracil auxotroph, Dikaryon