枯草芽孢杆菌无标记遗传操作技术研究进展

Marker-free genetic manipulation technologies in Bacillus subtilis

摘要:

枯草芽孢杆菌是革兰氏阳性菌的模式生物，长期以来在代谢工程和工业微生物领域扮演重要角色。枯草芽孢杆菌无标记遗传操作技术对后基因组时代的基因功能研究和菌株生理特性的改造起着关键作用。综述枯草芽孢杆菌无标记遗传操作所使用的负筛选标记基因，总结目前主流的无标记遗传操作的策略，并提出枯草芽孢杆菌无标记遗传操作技术面临的主要问题和发展方向。

Abstract:

Bacillus subtilis has been a model organism for gram-positive bacteria and has played important roles in metabolic engineering and industrial microbiology. Genetic manipulation technologies in Bacillus subtilis are critical for investigation of gene functions and modulation of bacteria physiologies in post-genomic era. In this review, we firstly summarize commonly used counter-selection markers for marker-free genetic manipulation. Then, we describe and compare different marker-free genetic manipulation strategies. Finally, we discuss challenges and future trends for developing more efficient tools for genetic manipulation of Bacillus subtilis.