The cyclic lipopeptides (CLPs) produced by Pseudomonas were made up of a cyclized oligopeptide lactone ring coupled to a fatty acid tail. CLPs were synthesized by nonribosomal peptide synthetases in Pseudomonas and the genetic regulation of CLPs syntheses was strict and comprehensive. GacA/GacS two-component signal transduction system and N-AHL-mediated quorum sensing system played an important role in global regulation of CLPs production in Pseudomonas. Here, we reviewed some research advances on the systems involved in CLPs syntheses. We discussed the method using PCR to obtain some cyclic lipopeptides and prospected the applications of bioengineering technology in enhancing the production of CLPs in Pseudomonas. It was also discussed the genome mining combining with genetic regulation research to obtain new cyclic lipopeptides.
