响应面法优化灰霉病生防菌CNY-04培养条件

Optimization of culture conditions of the biocontrol strain CNY-04 against Botryis cinerea by response surface methodology

摘要:

【目的】通过优化生防菌CNY-04的培养条件, 提高其对灰霉病菌的抑菌效果。【方法】在单因素试验的基础上, 利用响应面法(Response surface methodology)对灰霉病生防菌CNY-04培养条件进行整体优化, 并测其生长曲线。【结果】生防菌CNY-04最优培养条件为牛肉膏0.5%、蛋白胨2.0%、酵母膏0.1%、葡萄糖0.5%、时间48 h、接种量4%、温度32 °C、pH 8.0、装液量75 mL/250 mL和转速150 r/min, 在此培养条件下生防菌CNY-04的OD600为2.907, 与模型预测值相符, 对灰葡萄孢菌的抑菌圈直径为44.5 mm, 较优化前提高了30.9%。【结论】从整体上确定了生防菌CNY-04的最优培养条件, 为该菌扩大化生产提供理论基础。

Abstract:

[Objective] In order to raising the antagonistic effect, the growth conditions of biocontrol strain CNY-04 was optimized. [Methods] The growth conditions of the biocontrol strain CNY-04 were optimized by response surface methodology basing on single factor tests. The growth curve was also measured. [Results] The optimal culture conditions of the biocontrol strain CNY-04 were determined for 0.5% glucose, 2.0% peptone, 0.1% yeast extract, 0.5% beef extract, temperature at 32 °C, pH 8.0, speeding at 150 r/min, the incubation time of 48 h, inoculation amount of 4%, liquid volume of 75 mL/250 mL. The OD600 of CNY-04 was 2.907 with the optimal growth conditions, consistenting with the predicted value. The antibacterial circle diameter was 44.5 mm, improving by 30.9%. [Conclusion] The optimal growth condition of the biocontrol strain CNY-04 was identified, providing a theoretical basis for enlargement of production.