小麦印度腥黑穗病菌和黑麦草腥黑粉菌检测标准分子构建

Construction of Two Standard Molecules for Detection of Tilletia indica Mitra and Tilletia walkeri Castl.

摘要:

以小麦印度腥黑穗病菌和黑麦草腥黑粉菌为研究对象, 采用分子克隆技术, 分别构建了该两种真菌分子检测的标准分子。前者包括线粒体2297 bp的DNA序列以及rDNA 710 bp的ITS序列, 后者包括线粒体2.3 kb的DNA序列以及rDNA 710 bp的ITS序列。分别对该两个标准分子进行了性能评估试验, 测试结果显示所构建的标准分子具有良好的特异性、均匀性和稳定性, 能够满足小麦印度腥黑穗病菌和黑麦草腥黑粉菌分子检测需求。

关键词:克隆, 分子检测, 标准分子

Abstract:

Two standard molecules were constructed by molecular clone technology of Tilletia indica Mitra and Tilletia walkeri Castl.. The standard molecule for Tilletia indica Mitra contained two DNA fragments, one about 2297 bp DNA sequence from mitochondrial DNA, the other ITS sequence 710 bp. The standard molecule for Tilletia walkeri Castl. contained two DNA fragments, one about 2.3 kb DNA sequence from mitochondrial DNA, the other ITS sequence 710 bp. In this research, the two standard molecules were detected, the results show good specificity, homogeneity and stability which can satisfy the need of molecular detection of Tilletia indica Mitra and Tilletia walkeri Castl..

Key words:Clone, Molecular detection, Standard molecule