三种水生植物内生真菌多样性及其抗真菌活性

Diversity of Endophytic Fungi from Three Aquatic Plants and Their Antagonistic Activities in vitro

摘要:

从华凤仙(Impatiens chinensis L.)、问荆(Equisetum arvense L.)和轮叶狐尾藻(Myriophyllum verticillatum L.) 3种植物中共分离到内生真菌155株。经鉴定, 它们分属于26个不同的分类单元。以杨桃炭疽菌(Colletotrichum gloeosporioides)、香蕉疫霉(Phytophthora nicotianae)和腐皮镰刀菌(Fusarium solani)等6种植物病原真菌为指示菌, 对3种水生植物内生真菌代谢产物进行抗菌活性研究。结果表明, 有37株内生真菌(占分离菌株总数的23.9%)显示出对一种或多种病原菌的抑菌活性。来自华凤仙、问荆和轮叶狐尾藻的抗菌活性菌株比例分别为29.2%、13.9%及37.1%, 都远低于已报道的陆生植物内生真菌中活性菌株的比例。抗菌活性菌株主要分布于枝孢属(Cladosporium)、木霉属(Trichoderma)和地霉属(Geotrichum)等5个属中。

Abstract:

155 endophytic fungi were isolated from Equisetum arvense L., Impatiens chinensis L. and Myriophyllum verticillatum L. They were identified to 26 taxa. The antagonistic activities of these isolates against 6 isolates of plant pathogenic fungi were tested on the medium, and 37(23.9%) isolates were activitive against one or more phytopathogens. The percentage of antifungal active isolates from E. arvense L., I. chinensis L. and M. verticillatum L. were 13.9%, 29.2% and 37.1%, respectively. It was lower than that of terrestrial plants. The active isolates were mainly belonging to 5 genera including Cladosporium, Trichoderma and Geotrichum.