State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, Shanghai 200237, China 在期刊界中查找 在百度中查找 在本站中查找
State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, Shanghai 200237, China 在期刊界中查找 在百度中查找 在本站中查找
State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, Shanghai 200237, China 在期刊界中查找 在百度中查找 在本站中查找
State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, Shanghai 200237, China 在期刊界中查找 在百度中查找 在本站中查找
The lipopeptide samples were purified by normal reverse-phase column chromatography after isolated from the cell free broth of Brevibacillus brevis HOB1. A lipopeptide was separated from these samples by preparative HPLC, and determined to have a molecular weight of 1035.7 D by ESI-MS. The fatty acid part was identified to be C15 β-hydroxyl fatty acid by GC/MS. A quantitative analysis of the PITC-derivated amino acids of the lipopeptide by HPLC gave the following molar ratios: Asp 1, Glu 1, Val 1, Leu 4. All the results indicated that the lipopeptide has a structure similar to C15 surfactin. This study revealed that surfactin series lipopeptides can be produced by Brevibacillus genus in addition to Bacillus genus.
