HIV相关性口腔念珠菌RAPD多态性分析

Analysis on RAPD Genetic Polymorphism of HIV Related Oral Candida Species

摘要:

运用RAPD技术对60株分离自HIV感染者口腔的念珠菌进行分析, 其中P2随机引物扩增条带数量0~5条, 大小300 bp~2 kb, 白色念珠菌以300 bp、400 bp和600 bp 3个主条带为主, 非白色念珠菌也存在类似条带。采用SPSS13.0聚类分析, 分为5个基因群, 14个基因型, 白色念珠菌主要为B群。其中P385和P403对5-氟孢嘧啶耐药, 聚为C1基因型(欧氏距离平方为0.115), P321和P522对两性霉素B耐药, 聚为D1基因型(欧氏距离平方为0.221)。表明HIV感染者口腔来源的念珠菌存在丰富的基因多态性, RAPD技术对于白色念珠菌的基因型鉴定具有重要意义; 念珠菌不同种间具有相对特征性的条带, 同种不同株间存在相似的条带特征; 某些条带特征可能与念珠菌的耐药性相关。

Abstract:

60 Candida strains were analyzed with randomly amplified polymorphic DNA(RAPD) from HIV-infected patients’ oral cavity. The results showed that RAPD-PCR fingerprinting appeared from zero to five bands whose size rang from 300 bp to 2 kb with P2 primer, Candida albicans have three characteristic bands of 300 bp, 400 bp and 600 bp in size, non-Candida albicans have the similar bands. They can be classified five genetic clusters and 14 genotypes by Cluster Analysis, two Candida albicans(P385&P403) with fluorocytosine-resistance were classified into C1 genotype for “Squared Euclidean Distance” being 0.115, one Candida albicans(P321) and one Candida glabrata(P522) who were resistant against Amphotericin B were classified into D1 genotype for “Squared Euclidean Distance” being 0.221. So, an abundant genetic polymorphism appeared in Candida species related HIV, RAPD could be as a reference for genotype of Candida albicans; different Candida speices had some special bands, different Candida strains had similar bands in the same species; it is perhaps that some genotypes were relevant to drug resistance.