链霉菌Men-myco-93-63固体发酵的初步研究

Preliminary Study on Solid-state Fermentation of Streptomyces Strain Men-myco-93-63

摘要:

试验对链霉菌Men-myco-93-63固体发酵的培养基和培养条件进行了研究。以产孢量为指标, 通过单一固体基质筛选、组合固体基质筛选及正交试验得到适宜链霉菌Men-myco-93-63产孢的固体培养基是大米:高粱:米糠:稻壳为2:2:3:3(W/W/W/W), 试验中产孢量最高达到2.52×109 CFU/g。在此培养基的基础上研究了装料量、初始水料比、接种量和培养温度对其产孢的影响。结果表明, 链霉菌Men-myco-93-63产孢的适宜条件为：装料量为15 g/500 mL三角瓶, 初始水料比为1.7:1.0(V/W, 不计稻壳质量), 液体接种量为7 mL, 培养温度为28℃。

Abstract:

The culture medium and cultural conditions of solid-state fermentation of Streptomyces Men- myco-93-63 were tested in this study. The suitable medium which contains rice, sorghum, millet bran, and rice hull with the proportion of 2:2:3:3 was developed for the spore production of Streptomyces Men-myco-93-63 using single substrate screening, mixture substrate screening and orthogonal experiments, and the sporulation was up to 2.52×109 CFU/g. And then, initial charge, initial ratio of water to solid, inoculating quantity, and culture temperature impact to sporulation of Streptomyces Men-myco-93-63 were tested. The favorite cultural conditions are developed as the following: the initial charge is 15 g in 500 mL Erlenmeyer flask; initial ratio of water to solid is 1.7:1.0 (V/W, rice hull excluding), inoculating quantity is 7 mL, culture temperature is 28℃.