一组纤维素分解菌复合系NSC-7的酶活表达特性

Expression Characteristics of Enzyme Activity of a Multi-functional Bacterial Community NSC-7

摘要:

为了揭示一组具有降解纤维素和林丹双重功能的细菌复合系NSC-7的降解活性, 本文对该菌系的分解能力、纤维素酶活性和半纤维素酶活性进行测定。结果表明, NSC-7在14 d内, 可降解稻秆干重的 73.6％, 其中降解纤维素82.1％, 半纤维素58.2％, 木质素5.4％。用广泛采用的酶活测定方法测定了4种纤维素酶和半纤维素酶活性, 在培养的第8天, 内切酶、总纤维素酶、外切酶和b-糖苷酶活性都达到最大值, 分别为4.48 U/mL、7.51 U/mL、15.83 U/mL和25.78 U/mL。在培养的第5天, 半纤维素酶活性达到最高值为280.9 U/mL, 其平均值比纤维素酶活性高43.71倍。

Abstract:

In order to determine the degradation activity of the microbial community NSC-7, which had efficient cellulose and lindan degrading ability, degrading ability and four kinds of cellulase activity and hemicellulase activity were detected during degradation progress. The results showed that NSC-7 could degrade 73.6% of rice staw in weight, including 82.1% of cellulose, 58.2% of hemicellulase, and 5.4% of lignin within fourteen days. Endoglucanases, exoglucanases, β-glucosidases and the total cellulase activities reached to maximum at the eighth day, and they were 4.48 U/mL, 15.83 U/mL, 25.78 U/mL and 7.51U /mL, respectively. The maximum of hemicellulase activity was 280.6 U/mL at the fifth day, and the average of hemicellulase activity was 43.71 times higher than cellulase activity.