Abstract:Environmental Microbiology is a core course for undergraduates majoring in environmental science, playing an important role in the cultivating new quality talents under the background of emerging engineering education. In recent years, we have seriously reflected on the problems existing in the theory, practice and ideological and political aspects of the course of Environmental Microbiology, and carried out teaching reform by constructing a case library of curriculum ideological and political teaching, innovating the teaching contents, optimizing the teaching method and practical teaching contents, and reconstructing the course assessment system, so as to improve the ideological and moral quality and practical ability of students. After 3 years of exploration and practice of teaching reform, students’ achievement of course objectives is higher than expected, and the excellence rate in terminal evaluation is constantly improved. Moreover, students, peers, and supervisors give significantly improved evaluation results, which indicate that the teaching reform of this course has achieved satisfactory results.