Abstract:The main purpose of vocational education is to train technical talents who could apply their professional knowledge to production. It is necessary to improve the training of students’ practical skills and application ability. Microbiology is a core curriculum for students majoring in pharmaceutical biotechnology at Anhui Vocational and Technical College. Considering the learning situation and main teaching challenges, our teaching team has explored and reformed the teaching of Microbiology. We clarified the trinity teaching objectives of the curriculum through implementation of the new curriculum standards and reorganized the teaching contents to improve the practical skills of students based on job requirements. By combining reality with virtuality, theory with practice, and online and offline teaching, we built a student-centered efficient class incorporating ideological and political education. Furthermore, we built a multi-dimensional evaluation system for the entire teaching process to control the teaching effectiveness. The teaching reform fully mobilized the learning enthusiasm and initiative, clarified the study goals, and enhanced the comprehensive vocational abilities of students, thereby significantly improving the teaching effectiveness. It supports the training of qualified talents who are able to provide technical services.