Abstract:The core of new medicine is the training of compound medical talents, and the development of professional core curriculums provides all-round support for talent training. Medical Microbiology Experiment is a professional core course for clinical medicine and optometry medicine, playing an irreplaceable role in cultivating students’ consciousness of innovation, practical ability, and high-order thinking. Under the current teaching paradigm, students have difficulty in maintaining self-learning, weak interdisciplinary and translational application ability, lack of innovative thinking in scientific research, and poor internalization of curriculum ideology and politics, and teachers lack comprehensive consideration in the assessment and evaluation of students. In view of the problems above and under the background of new medicine, the teaching team in our university focuses on the development of students for years and establishes a “five-in-one” teaching system for Medical Microbiology Experiment from reconstructing multi-level teaching contents with characteristics of new medicine, integrating outcome-based education (OBE) into the ideological and political system, reforming the blended teaching mode, creating a new paradigm of collaborative teaching and research in and after the class, and building a “diversity+challenge” teaching evaluation system. This teaching system is suitable for training professional, innovative, and composite top-notch medical talents. The results show that the teaching reform plays a positive role in consolidating medical students’ professional knowledge and skills, stimulating their learning enthusiasm and creativity, training students to use artificial intelligence to solve medical problems, and improving medical humanistic spirit and professionalism. It helps to realize the goal of training compound medical talents under the background of new medicine and provides a paradigm and experience for the reform of experimental courses in medical colleges.