植物内生菌分离培养技术研究进展

Technological advances in isolation and culture of plant endophytes

摘要:

植物内生菌是指生活史的全部或部分阶段栖息于植物组织内的微生物类群。内生菌与植物协同进化，并在宿主的生长发育和环境适应中产生诸多效应。近30年来，针对内生菌的起源、传递、功能及其与宿主植物相互作用规律和机制方面已有大量的研究成果。然而，目前的技术手段局限性使得绝大多数的植物内生菌仍不能被纯培养，这严重制约了这些植物内生菌资源的研究和有效利用。本文综述了目前对内生菌特别是那些难培养内生菌分离培养方法的现状，着重关注植物内生菌分离培养方面的技术创新和进展，为植物内生菌的深入研究和资源的有效开发利用提供借鉴。

Abstract:

Plant endophytes are microorganisms that reside inside plant tissue for all or part of their life cycle. These endophytes have co-evolved with plants, contributing significantly to host growth, development, and environmental adaptation. Over the past 30 years, extensive research has been conducted on the origins, transmission, and functions of plant endophytes and the interaction mechanisms between endophytes and their host plants. However, current technological limitations hinder the pure culture of the majority of endophytes, restricting the study and utilization of these valuable microbial resources. This paper provides an overview of current methods for isolating and culturing plant endophytes, with a particular focus on cultivation-recalcitrant endophytes (CREs), highlighting recent technological innovations and advances in this field to offer insights for in-depth research and effective exploitation of endophyte resources.