9781.
总被引：1，自引：0，他引：1
<正>透明嗜热腐殖霉菌图1,2 Humicola hyalothermophila Moubasher,Mazen & Abdel-Hafez,Transactions of the British Mycological Society,72:509,1979.Fig.1,2在不同温度的PDA培养基上培养5d,19℃下无生长现象;45℃生长迅速;50℃生长较为缓慢。菌… … 相似文献
9782.
总被引：1，自引：0，他引：1
报道了海南岛境内的灵芝科真菌4种。其中1新种即尖峰岭灵芝Ganoderma jianfenglingense X.L.Wu sp.nov。岛内新记录有3种即薄盖灵芝Ganoderma capense(Lloyd)Teng;大青山灵芝G.daiqingshanense Zhao;褐… … 相似文献
9783.
9784.
总被引：1，自引：1，他引：1
肉疣衣属地衣为世界广布属之一,该属为壳状地衣,地衣体通常龟裂具疣,子囊果盘状一般无柄贴生,盘缘明显,侧丝分枝结网状,孢子大型。本文对中国肉疣衣属地衣进行了初步研究,并报道了该属地衣共16种,其中中国新记录5种,即寒生肉疣衣、柱芽肉疣衣、粉末肉疣衣、亚裂芽肉疣衣、亚绿肉疣衣。… … 相似文献
9785.
9786.
总被引：1，自引：0，他引：1
为了比较糙皮侧耳栽培种在不同常规栽培基质上的漆酶活性,分析更适合糙皮侧耳生长的栽培基质,以1株糙皮侧耳Pleurotus ostreatus栽培菌株为研究材料,研究在固态和液态发酵条件下添加木屑、玉米芯和棉籽壳这3种栽培基质后,对其产漆酶活性的影响。结果表明,不同栽培基质对糙皮侧耳漆酶活性具有极显著的影响（P<0.001）;不同发酵方法对糙皮侧耳漆酶活性也具有极显著的影响（P<0.001）,仅第2天差异不显著。固体发酵与液体发酵条件下,糙皮侧耳在棉籽壳培养基上所检测到的漆酶活性均高于在木屑或者玉米芯培养基上,表明棉籽壳对提高糙皮侧耳漆酶活性的诱导能力更强。此外,糙皮侧耳在棉籽壳培养基上能够快速分泌漆酶,表明棉籽壳对缩短糙皮侧耳漆酶分泌时间的诱导能力更强。… … 相似文献
9787.
总被引：1，自引：1，他引：1
苜蓿中华根瘤菌(Sinorhizobiummeliloti) 0 4 2BM与耐盐有关的1 9kbDNA片段含有两个开放阅读框,采用PCR方法分别将它们扩增,连接到穿梭质粒上,并进行了耐盐功能检测,证明其中的ORF2具有耐盐性,定名为rstA基因。将它分别克隆到表达载体pThio… … 相似文献
9789.
9790.
总被引：1，自引：0，他引：1
以人胎肝ｍＲＮＡ为模板,采用ＲＴ－ＰＣＲ扩增了人ＴＰＯ编码区全长ｃＤＮＡ,全序列测定结果表明与国外报道序列一致;进而构建了ｐｃＤＮＡ３－ＴＰＯ永久表达载体,转染ＣＨＯ细胞后经Ｇ４１８加压筛选、ＴＰＯ表达稳定性等项指标测试,获得了稳定分泌ＴＰＯ的工程细胞株。 相似文献
9791.
9792.
9793.
9794.
总被引：1，自引：0，他引：1
A geldanamycin (GDM)-producing strain, Streptomyces hygroscopicus 17997, was isolated from the soil of Yunnan, China, by the researchers of the Institute of Medicinal Biotechnology, CAMS & PUMC. GDM is an ansamycin antibiotic, which has the ability to bind with Hsp90 (heat shock protein 90) and alter its function. Hsp90 plays a key role in regulating the physiology of cells exposed to environmental stress and in maintaining the malignant phenotype of tumor cells. As an inhibitor of Hsp90, GDM possesses potent antitumoral and antiviral bioactivity, but the hypatotoxicity and poor solubility in water limit its clinical use. To accomplish the structural modification of GDM by genetic means, an attempt to obtain the biosynthetic gene cluster of GDM from S. hygroscopicus 17997 was made. In this study, a pair of primers was designed according to a conserved sequence of one of the possible post-PKS (polyketides synthase) modification genes, the carbamoyltransferase (CT) gene (gdmN) in GDM biosynthesis. The 732-bp PCR product was obtained from the S. hygroscopicus 17997 genomic DNA. Through the colony-PCR Binary Search Method, using the CT gene primers, six positive cosmid clones, CT1-6, were identified from the S. hygroscopicus 17997 cosmid genomic library. The CT-4 positive cosmid was then sub-cloned and sequenced. Approximately 28.356 kb of foreign gene sequence from CT-4 cosmid and by further PCR extension reaction was obtained. According to BLAST analysis, this sequence contains 13 possible ORFs, and they are believed to be involved in GDM production. The obtained possible GDM biosynthetic gene cluster in S. hygroscopicus 17997 will facilitate the further functional analysis of the genes and the modification of the structure of GDM through combinatorial biosynthesis.… … 相似文献
9795.
9796.
9797.
9798.
9799.
9800.