Yeast surface display system: optimization strategies and application in high-throughput screening

Abstract:

Yeast surface display technology, which enables the expression and display of target proteins on the surface of yeast cells, has been successfully constructed in various yeast species like Saccharomyces cerevisiae, Pichia pastoris, and Yarrowia lipolytica. This technology provides highly efficient platforms for protein high-throughput screening, enabling rapid screening and evaluation of pharmaceutical and antibody candidates, as well as recombinant strains with improved secretion efficiency. It has been widely applied in biopharmaceutical production, vaccine development, enzyme engineering, and biomaterial production, significantly contributing to advancements in biotechnology. This review delves into optimization strategies for yeast surface display and its applications in high-throughput screening. In addition, it discusses current limitations and explores future directions of this technology, laying the groundwork for enhancing the accuracy and enlarging the scope of applications.