乳酸片球菌HRQ-1微胶囊的制备、优化及其体外评估

Preparation, optimization, and in vitro evaluation of Pediococcus acidilactici HRQ-1 microcapsules

摘要:

为提高本课题组前期筛选得到的一株肠道益生菌——乳酸片球菌(Pediococcus acidilactici) HRQ-1的胃肠液耐受能力、运输储存稳定性以及缓慢释放性能，本研究以乳酸片球菌为芯材，海藻酸钠和壳聚糖为壁材，采用挤压法制备乳酸片球菌微胶囊。通过单因素和正交试验确定乳酸片球菌微胶囊的最佳制备工艺条件，并以包埋率、存活率、储存稳定性、肠胃液耐受性及释放速率为评定指标，对乳酸片球菌微胶囊最佳配比进行研究。结果表明，在乳酸片球菌微胶囊最佳包埋条件下，包埋率达到(89.60±0.02)%；在最佳的冻干保护剂配方条件下，冻干存活率达到(76.42±0.13)%，制得的微胶囊粒径平均值为(1.16±0.03) mm；通过连续胃肠液模拟实验证实乳酸片球菌微胶囊活菌数得到保证且可在肠液中缓慢释放，通过4℃与室温储存过程活菌数量变化曲线可知制备该微胶囊可实现菌株有效活菌数的保障。本研究中乳酸片球菌微胶囊的制备配方与工艺为活菌药物制备提供技了术支持。

Abstract:

We have isolated an intestinal probiotic strain, Pediococcus acidilactici HRQ-1. To improve its gastrointestinal fluid tolerance, transportation and storage stability, and slow-release properties, we employed the extrusion method to prepare the microcapsules with P. acidilactici HRQ-1 as the core material and sodium alginate and chitosan as the wall material. The optimal conditions for preparing the microcapsules were determined by single factor and orthogonal tests, and the optimal ratio was determined by taking the embedding rate, survival rate, storage stability, gastrointestinal fluid tolerance, and release rate as the evaluation indexes. The results showed that under the optimal embedding conditions, the embedding rate reached (89.60±0.02)%. Under the optimal formula of freeze-drying protective agent, the freeze-drying survival rate reached (76.42±0.13)%, and the average size of the microcapsules produced was (1.16±0.03) mm. The continuous gastrointestinal fluid simulation experiments confirmed that the microcapsules ensured the viable bacterial count and can slowly release bacteria in the intestinal fluid. The curve of the viable bacterial count during storage at 4 ℃ and room temperature indicated that the prepared microcapsules achieved strains’ live number protection. The formula and preparation process of P. acidilactici microcapsules may provide a technological reserve for the preparation of more live bacterial drugs in the future.