酵母中性位点的挖掘及应用研究进展

Research progress in the mining and application of neutral sites in yeast

摘要:

酵母是代谢工程中生产蛋白质、化学品和植物天然产物的重要宿主，挖掘多样化的酵母中性位点对复杂产物的高效细胞工厂的构建和优化具有重要的意义。本文归纳总结了酵母染色体上中性位点的筛选标准、表征手段、强度影响因素，介绍了其在合成生物学和生物制造领域中的应用，并对未来的研究方向进行了展望，为利用中性位点进行酵母细胞工厂的构建优化提供了理论支撑。

Abstract:

Yeast is a valuable host organism for protein, chemical, and plant natural product production in metabolic engineering. The development of neutral sites in yeast holds great significance for the construction and optimization of efficient cell factories. This review summarizes the screening standards, characterization methods, influencing factors, and applications of neutral sites in synthetic biology. Additionally, it discusses the future research directions, providing theoretical support and practical guidance for the construction and optimization of yeast cell factories utilizing neutral sites.