酵母表面展示系统的优化策略及其在高通量筛选中的应用

Yeast surface display system: optimization strategies and application in high-throughput screening

摘要:

酵母表面展示是一种将目标蛋白在酵母细胞表面表达的技术，已在酿酒酵母、毕赤酵母、解脂耶氏酵母等多个酵母种属中成功建立。该技术为蛋白高通量筛选提供了高效的平台，用于对药物和抗体等候选分子以及高分泌细胞进行快速和准确的评估，已在生物制药、疫苗开发、酶工程和生物材料制备等关键领域广泛应用。本文综述了酵母表面展示技术中针对宿主和表达盒元件的优化策略，并深入探讨了该技术在高通量筛选中的应用。同时，也指出了当前技术的局限性，并对未来的发展方向进行了展望，为进一步提升筛选的准确性和扩大适用范围提供了参考。

Abstract:

Yeast surface display technology, which enables the expression and display of target proteins on the surface of yeast cells, has been successfully constructed in various yeast species like Saccharomyces cerevisiae, Pichia pastoris, and Yarrowia lipolytica. This technology provides highly efficient platforms for protein high-throughput screening, enabling rapid screening and evaluation of pharmaceutical and antibody candidates, as well as recombinant strains with improved secretion efficiency. It has been widely applied in biopharmaceutical production, vaccine development, enzyme engineering, and biomaterial production, significantly contributing to advancements in biotechnology. This review delves into optimization strategies for yeast surface display and its applications in high-throughput screening. In addition, it discusses current limitations and explores future directions of this technology, laying the groundwork for enhancing the accuracy and enlarging the scope of applications.