罗氏真养产碱杆菌代谢工程研究进展

Research progress in metabolic engineering of Cupriavidus necator

摘要:

罗氏真养产碱杆菌(Cupriavidus necator)是一种兼性化能自养细菌，属于变形菌门(Proteobacteria) β变形菌亚纲，该菌能利用CO₂化能自养生长，生产聚羟基脂肪酸酯(polyhydroxyalkanoates, PHA)等产物。在全球碳减排的背景下，该菌在利用CO₂生产有机酸、PHA及次级代谢产物等关键生物基产品方面有望发挥重要作用。自20世纪90年代代谢工程理念提出以来，尤其是代谢工程使能技术的创新及发展，极大地促进了C.necator细胞工厂的构建及其在工业发酵领域的应用。本文系统介绍了近年来C. necator H16代谢工程技术的发展，及其在生物基化学品细胞工厂构建中的应用，最后讨论了C.necator H16代谢工程中关键问题并展望其未来发展前景，可为增强C. necator H16代谢工程的研究提供思路，促进其在二氧化碳转化和生物制造中更广泛的应用。

Abstract:

Cupriavidus necrotor is a facultative autotrophic bacterium belonging to Betaproteobacteria. It can grow and produce PHA and other products using CO₂. This bacterium has received widespread attention in the context of global CO₂ reduction and is believed to play an important role in the production of key bio-based products such as organic acids, PHA, and secondary metabolites using CO₂. The proposal of metabolic engineering in the 1990s, especially the innovation and development of metabolic engineering enabling technologies, has greatly promoted the construction of C. necator cell factories and the application of the cell factories in industrial fermentation. The article systematically introduces the recent progress in the metabolic engineering of C. necator H16 and the construction of cell factories for producing bio-based chemicals. Finally, the key issues and future development prospects in the metabolic engineering of C. necator H16 are discussed. The purpose of the article is to offer valuable insights and research ideas to the metabolic engineering studies of C. necator H16, and therefore, promote more extensive applications of this strain in carbon dioxide conversion and bio-manufacturing.