CRISPR/Cas基因编辑和表达调控工具箱在放线菌中的应用

CRISPR/Cas toolkit for gene editing and expression regulation in actinomycetes

摘要:

放线菌是一类具有强大次级代谢能力的微生物，对放线菌菌株进行理性改造，对提升其天然产物的生产能力具有重要意义。CRISPR/Cas系统因具有高效性、易操作性和良好的靶向精确性，已成为放线菌基因改造和功能研究的重要工具。本文从工作原理、系统构建、适用场景等角度对放线菌中的各类CRISPR/Cas基因编辑及表达调控工具进行了汇总和比较，包括基于DNA双链断裂的CRISPR/Cas系统、CRISPR干扰系统、CRISPR激活系统，以及CRISPR碱基编辑系统。同时，本文还总结了这些工具在放线菌天然产物生物合成方面的应用情况。CRISPR/Cas工具箱在放线菌中的开发和应用，极大地推动了放线菌微生物学和天然产物相关的研究。

Abstract:

Actinomycetes are a class of microorganisms with robust secondary metabolism. Rationally engineering actinomycete strains to enhance their production of natural products is of significant importance. The CRISPR/Cas system, known for its high efficiency, ease of operation, and excellent targeting precision, has become a vital tool for genetic modification and functional studies in actinomycetes. This article provides a comprehensive overview and comparison of various CRISPR/Cas tools for gene editing and expression regulation in actinomycetes, including DNA double-strand break-based CRISPR/Cas systems, CRISPR interference systems, CRISPR activation systems, and CRISPR base editing systems. In addition, this article summarizes the applications of these tools in the biosynthesis of natural products in actinomycetes. The development and application of the CRISPR/Cas toolkit in actinomycetes have greatly promoted the research of microbiology and natural products of actinomycetes.