噬菌体免疫沉淀测序技术研究进展

Advances in phage immunoprecipitation sequencing technology

摘要:

噬菌体免疫沉淀测序技术(phage immunoprecipitation sequencing, PhIP-Seq)是一种高通量、低成本分析目标蛋白与肽库特异性结合的方法。该方法使用寡核苷酸文库合成(oligonucleotide library synthesis, OLS)来编码蛋白质组规模的肽库以展示在噬菌体上，然后使用目标蛋白(抗体等)对这些文库噬菌体进行免疫沉淀，以便随后通过高通量DNA测序进行分析。通过PhIP-Seq可以筛选出与数百种蛋白质或病原体特异性反应的肽靶标。PhIP-Seq已成功应用于疾病检测、自身免疫性疾病生物标志物的筛选、疫苗开发和过敏原检测等领域，是一项重要的高通量诊断技术。本文系统地阐述了噬菌体免疫沉淀测序的发展、应用和结果评估，有助于研究人员更全面地了解该技术在各领域的应用和未来发展的前景。

Abstract:

Phage immunoprecipitation sequencing (PhIP-Seq) is a high-throughput and low-cost method for analyzing the specific binding of target proteins to peptide libraries. The method uses oligonucleotide library synthesis (OLS) to encode proteome-scale peptide libraries for display on phages, and then immunoprecipitates these library phages with target proteins (such as antibodies) for subsequent analysis by high-throughput DNA sequencing. PhIP-Seq enables the screening of peptide targets that react specifically with hundreds of proteins or pathogens. PhIP-Seq has been successfully applied in various fields such as disease detection, screening of autoimmune disease biomarkers, vaccine development, and allergen detection, becoming a high-throughput diagnostic technology. This article systematically describes the development, applications, and result evaluation of PhIP-Seq, in order to gain a more comprehensive understanding of the application and future development prospects of this technology in various fields.