Sequence analysis for the complete proviral genome of Avian Leukosis Virus（Subgroup J）strain SD07LK1 isolated from layers

Abstract:

Abstract: [Objective]To study the source and trend in evolution of ALV-J strains from layers in China. [Methods]The genomic DNA extracted from chicken embryo fibroblasts (CEF) infected by ALV-J strain SD07LK1 isolated from layers was used as template to amplify the ALV-J proviral DNA by PCR. Nine continuous and overlapping fragments were amplified with nine pairs of primers according to published sequences, then cloned into the T vector and sequenced. [Results]The complete sequence of the whole genome of ALV-J Chinese strain SD07LK1 was first established. [Conclusion]Comparisons of SD07LK1 sequence with that of the other Avian leukosis viruse strains, by using DNAstar software, demonstrated that the genes gag and pol of ALV-J were relatively conservative, the nucleotide identity of all the strains was over 95.0%. However, the gene env identity was only in the ranges between 88.6 and 94.0%.