Ferric reductase FRP1 gene in Candida albicans

Abstract:

Abstract: The ability of iron acquisition in Candida albicans has an effect on its growth and pathogenesis. Ferric reductases are important components of high affinity iron acquisition system in C. albicans. [Objective] The aim of this study was to elucidate the function of FRP1 (Ferric reductase protein). [Methods] FRP1 gene expression was detected under low-iron and high-iron conditions by Northern blot. We used the method of PCR-directed gene disruption to construct frp1 null mutant and then the phenotypes of frp1Δ/Δ mutant were characterized. [Results] Low-iron condition induced FRP1 gene expression. frp1Δ/Δ mutant showed no growth on low-iron media compared to wild-type strains on solid plates. [Conclusion] FRP1 protein is probably the main ferric reductase under low-iron condition.