Analysis of Legionella pnumophlia isolated from Guangdong province by amplified fragment length polymorphism protocol

Abstract:

[Objective] To analyze the genetic relationship and predominate genotypes among Legionella pneumophila isolated from Guangdong province. [Methods] In total 43 Legionella pneumophila strains were genotyped according to the amplified fragment length polymorphism protocol, and the obtained electropherograms were contrasted to the published standard patterns of the European Working Group for Legionella Infections. [Results] In total 33 genotypes were identified from those 43 Legionella, the discriminative index was 99.79%, and the Kingmed AFLP type no. 011 was predominate. In total 18 clusters were discriminated by 0.8 dice coefficient, and the Kingmed AFLP cluster D was predominate. By comparison, 7 respective genotypes had high similarity to the EWGLI AFLP type no. 001 Lugano, 002 Lugano, 012 Rome, 013 London, 014 London, 015 Dresden and 021 Lyon, and it also existed some potential new genotypes. [Conclusion] The genetic polymorphism of Legionella pneumophila are diverse in the environmental water of Guangdong province, and amplified fragment length polymorphism is one of the effective means for the research of molecular epidemiology.