A multi-frequency large amplitude pulse sensor system to monitor the growth of food-borne pathogens

Abstract:

[Objective] We explored whether a smart electronic tongue as a rapid technique, combining with specifically statistics analyze system, could monitor the growth of the three food-borne pathogens. [Methods] The smart electronic tongue, multi-frequency large amplitude pulse was based on voltammetry .We used it to detect the comprehensive information about a sample and the change process of the medium in 16 periods of time. We analyzed the complex data information in statistics with principal component analysis. According to principal component scores plot, we analyzed the sample. [Results] The growth of Staphylococcus aureus in liquid medium could be monitored by tungsten electrode in 100 Hz frequency segment of multi-frequency large amplitude pulse voltammetry, platinum electrode in 1 Hz, and both silver electrode and titanium electrode in 10 Hz. The growth of Escherichia coli O157:H7 could be measured by gold electrode in 100 Hz frequency segment, while platinum electrode in 1Hz, titanium electrode in 1Hz and tungsten electrode in 100 Hz. Then, palladium electrode was efficient to Bacillus subtilis in 1 Hz, 10 Hz, 100 Hz. [Conclusion] We used the multi-frequency large amplitude pulse sensor system combining with principal component analysis for the first time. It has a promising future as a modern rapid analytical technology for detecting the growth of microbe.