Cloning and bioinformatic analysis of calcineurin B gene inSetosphaeria turcica

Abstract:

[Objective] To clone and identify CnB gene from Setosphaeria turcica and to perform the primary bioinformatic analysis. [Methods] Using RACE method, we isolated the completed cDNA sequence and DNA sequence of CnB gene from S. turcica. Corresponding structure and functions were predicted by the bioinformatic software. [Results] The results showed that CnB gene had 4 exons and 3 introns and the largest open reading frame was 525 bp which encoded a protein of 174 amino acid residues. The predicted secondary structure composition for the protein contained about 59.77% alpha helixes, 8.62% beta turn, 6.32% extended strand and 25.29% random coil. The CnB gene of S. turcica had 4 “EF-hand” calcium-binding regions, and showed 90% homology with CnB gene of P. nodorum, B. cinerea and Neurospora crassa. [Conclusion] The CnB gene was successfully cloned from S. turcica for the first time, providing a good foundation for further research on its function.