College of Food & Biotechnology Engineering, Zhejiang Gongshang University, Hangzhou 310035, China

Abstract:

[Objective] To enhance the production of plantaricin by Lactobacillus plantarum ZJ316 isolated from infant feces. [Methods] We analyzed fermentation parameters influencing cell growth and plantaricin production with different medium composition and under different cultivation conditions. [Results] MRS (DE MAN, ROGOSA, SHARPE) medium was more suitable for producing bacteriocin than other media. The maximum plantaricin production was obtained in modified MRS medium containing 10 g/L maltose and 10 g/L glucose, 10 g/L yeast extract, 10 g/L tryptone and 2g/L tri-ammonium citrate, 1 mL/L Tween80, 6 g/L K2HPO4 ? 3H2O, 5 g/L sodium acetate, 0.2 g/L magnesium sulfate and 0.05 g/L manganese sulfate. The optimal initial pH and temperature for plantaricin production were 6.5 and 30℃ for 24 h. [Conclusion] After optimizing, the production of plantaricin was increased 2.3-fold using the optimized medium, compared with in the basic MRS medium.