An improved replica plating method and its application to the high-throughput selection of transformants

Abstract:

[Objective] To make a simpler and highly efficient replica plating tool to avoid the limitations of the traditional method. [Methods] A number of bamboo-toothpicks was sheaved to form a bundle with diameter close to the plate diameter. The sheaved bamboo-toothpicks were used for replica-plating. [Results] This replica plating tool was successfully used for the screening of Saccahromyces cerevisiae transformants. [Conclusion] Compared with the traditional tool, this tool was simpler and highly efficient.