Genus-specific PCR for molecular identification of novel isolates of the genus Nesterenkonia

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Genus-specific PCR for molecular identification of novel isolates of the genus Nesterenkonia
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Fund Project:Supported by the Key Project of China National Programs for Fundamental Research and Development (2004CB719601), the National Natural Science Foundation of China (30600001), the Key Project of Chinese Ministry of Education (206139) and the Program for New

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[Objective and Method]: For rapid identification of Nesterenkonia species, one set of genus-specific primers was designed and synthesized for polymerase chain reaction based on the 16S rRNA gene sequences. [Results and Conclusion]: The genus specificity of these primers was validated with reference strains as well as with wild-type isolates. Partial sequencing results of 16S rRNA gene of the wild-type isolates confirmed that they are members of the genus Nesterenkonia.

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Xiaoyang Zhi, Lingling Yang, Yun Wang, Shukun Tang, Kai Lou, Wenjun Li. Genus-specific PCR for molecular identification of novel isolates of the genus Nesterenkonia. [J]. Acta Microbiologica Sinica, 2008, 48(5): 644-650

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Received:November 12,2007
Revised:December 27,2007
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