Transduction of rhesus bone marrow mesenchymal stem cells by recombinant baculovirus

Abstract:

[Objective] To investigate whether the recombinant baculovirus (Bac-CMV-EGFP) can effectively transduce into rhesus Bone-marrow derived Mesenchymal Stem Cells (rBMSCs) in vitro, and whether there are some efficiency to the rBMSCs of viability, proliferational and differentiational capacity after recombinant baculovirus transducing. [Methods] The rBMSCs were cultured in vitro. After passaged more than three times, the rBMSCs were transduced with various dose of baculovirus (Multiplicity Of Infection, MOI, the MOI is 50, 100, 200, 300, and 500 vector genome (vg)/cell, re-spectively). We used flow cytometry to detect different transductive efficiency of various dose of baculovirus to rBMSCs. Under a suitable dose of baculovirus (300v.g/cell), we studied cell viability, proliferation and differentiation capacity, and compared results with the control. [Results] Baculovirus could be transduced into rBMSCs in vitro. The transductive ef-ficiency reached about 80% when the MOI was 300v.g/cell, at 25℃, and incubated for 4 h. Furthermore, under a higher transductive efficiency of baculovirus, there were no obvious influence to the rBMSCs of viability, proliferation and dif-ferentiation capacity compared with that of the control. [Conclusion] The baculovirus can be safely and effectively transduced into rBMSCs in vitro, without any negative efficiency to cell viability, proliferation and differentiation capacity.