Isolation,identification and characterization of nitrate-reducing and sulfide-oxidizing bacteria

Abstract:

Bacterial strains CB and CS were isolated from the Anoxic Sulfide Oxidizing (ASO) reactor working under steady-state with sulfide and nitrate as electron donor and acceptor，respectively. Based on electron microscopy，physiological test and 16S rDNA phylogenetic sequence analysis，the isolate CB was very close to Bacillus pseudofirmus and the isolate CS was very close to Bacillus hemicellulosilytus and Bacillus halodurans. The 16S rDNA sequences of both isolates were submitted to GenBank，NCBI and accession numbers EF542806 and EF542807 were allotted for isolates CB and CS，respectively. According to Biolog carbon source utilization test，the isolate CB is weak to use the carbon sources，while the isolate CB can use many carbon sources. Both the isolate CB and the isolate CS are able to use nitrate for sulfide oxidation. The isolate CS has greater capability to oxidize sulfide with nitrate as electron acceptor.