Expression of recombinant bovine beta-interferon by baculovirus system and evaluation of its bioactivity

Abstract:

In this paper，a recombinant baculovirus containing the ORF of bovine interferon-β (BoIFN-β) gene，rBac-BoIFN-β，was generated to express recombinant BoIFN-β (rBoIFN-β) in sf9 insect cells. The expression of rBoIFN-β in rBac-BoIFN-β infecting sf9 cells and its supernatants was confirmed by indirect immunofluorescence assay and Western blot. The antiviral activity of rBoIFN-β in the supernatant can reach 10^6.0AU/mL evaluated by the antiviral assay with VSV*GFP that expressed green fluorescence protein，and rBoIFN-β could stimulate the expression of luciferase reporter gene controlled by chicken Mx promoter. All the results showed that rBac-BoIFN-β constructed here could express high level recombinant BoIFN-β in secreted form that had the bioactivity of natural type Ⅰ IFN.