Extraction of mitochondrial DNA of Tilletia indica Mitra and application of the ATP6 gene on fungal phylogenetic analysis

Abstract:

Tilletia indica Mitra，an important pest around the world which is the causative agent of Karnal Bunt of wheat，was very close to Tilletia walkeri phylogenetically and morphologically. 69.6ng mitochondrial DNA (mtDNA) of Tilletia indica obtained from 6.4mg total DNA by the method of CsCl/bisbenzimide density gradient ultracentrifugation can be used for electrophoresis，cloning，enzyme restriction analysis and PCR amplification. The ATP6 gene sequence was cloned from the fragment of mtDNA and sequenced for analysis of phylogenetic tree with the other related sequences in GenBank by the software PAUP to reveal the phylogenetic relationships within the Tilletia species.