Diversity of associated nitrogen-fixing bacteria isolated from the pioneer plants-Vetiver zizanioides

Abstract:

Abstract: [Objective] Vetiver zizanioides is a perennial grass of the Poaceae family, known as silage, soil and water conservation role. The aim is to collect and identify the resources of the nitrogen-fixing bacteria associated with Vetiver zizanioides. [Methods] Associated nitrogen-fixing bacteria isolated from Vetiver zizanioides, were studied by SDS-PAGE whole-cell protein patterns, insert sequence (IS)-PCR finger printing, utilization of sole carbon sources and 16S rRNA gene sequence analysis. [Results] Based on the results of finger printing analysis, protein patterns and biological test, isolates were grouped into 6 clusters, except 4 single strains. Phylogenetic analysis of 16S rDNA sequences indicated that isolates belonged to Herbaspirillum frisingense, Enterobacter ludwigii, Pseudacidovorax intermedius, Mitsuaria chitosanitabida, Pseudomonas putida, Pseudomonas fluorescens, Burkholderia vietnamiensis and Enterobacter cloacae. [Conclusion] The nitrogen fixers associated with Vetiver zizanioides showed great diversity and may have a potential application for the grass forage and agriculture.