Anti-tumor compounds from submerger fermentation products of a Paecilomyces militaris strain

Abstract:

Abstract: [Objective] The aim was to identify the anti-tumor compounds of the submerger fermentation broth of Paecilomyces militaris (strain RCEF0927). [Methods] Anti-tumor activity was tested with a resazurin cytotoxicity assay model with Chinese hamster ovary (CHO) cells. Bioactive compounds were analysed with combined method of High Performance Liquid Chromatography (HPLC), High Resolution Mass Spectrometer (HRMS) and anti-tumor assay. [Results] Activity test showed that the submerger fermentation broth had strong cytotoxicity against CHO cells. Extraction tests showed that ethylacetate was the best solvent for the bioactive constituents extracting. HPLC-DAD- HRMS-Cytotoxicity assay revealed that the molecular formulars of the active compounds in the extract were possibly C10H13N5O3, C22H22O9, C23H24O10, C22H22O10, C15H10O4, C15H10O, C36H70O11, C36H68O12, C38H74O11, C40H76O13 and C44H82O14. [Conclusion]The possible structures of the bioactibe compounds were deduced with bioassay, HRMS, Uv and database inquiry as cordycepin, daizein, genistein 7,4′-dihydroxyisoflavone-7-O-(4″-O-methyl)-?-D-glucopyranoside, 7,4′-dihydroxy-6- methoxyisoflavone-7-O-(4″-O-methyl)-?-D-glucopyranoside, 5,7,4′-trihydroxy- isoflavone-7-O-(4″- O-methyl)- ?-D- glucopyranoside, polyoxyethylene ether oleate, dehydro products of polyoxyethylene sorbitan monostearate, polyoxyethylene sorbitan monooleate, polyoxypropylene or polyoxybutene contained derivants of polyoxyethylene sorbitan fatty acid ester. Cytotoxicity of the compounds was revealed for the first time.