Acid and bile tolerance and cholesterol reduction ability of Lactobacillus paraplantarum
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Supported by the National Natural Science Foundation of China(30671398) and the NSFC-KOSEF Joint Research Projects(30711140389)

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    Abstract:

    Abstract: [Objective] We studied the acid and bile tolerance of Lactobacillus paraplantarumⅡ32, and examined the possible mechanisms of cholesterol reduction. [Methods] StrainⅡ32 was incubated in MRS (de Man, Rogosa, Sharpe) broth supplemented with cholesterol, and the cholesterol in medium was determined using gas chromatography. We examined cholesterol reducing ability under different conditions, and the relation between growth and cholesterol reduction of strainⅡ32. [Results] StrainⅡ32 showed acid resistance, bile salt tolerance and the cholesterol- reducing ability. The results indicated that strainⅡ32 survived at pH2 after 2h culture and the living bacterial number could reach 104 cfu/mL. In addition, the growth of strainⅡ32 was delayed less than 0.5h in MRS broth with 0.3%-0.4% bile salt, when the absorbance value both increased to 0.6 unit. Cholesterol removed by dead and resting cells were 5.64 and 5.90 mg/g of dry weight compared with growing cells, which was 16.98 mg/g of dry weight. Cholesterol removal was greatly associated with growth of the bacteria. The possible mechanisms for removal of cholesterol from media were proposed: assimilation of cholesterol during growth, and binding of cholesterol to the cell surface. [Conclusion] StrainⅡ32 may be promising candidates for use as a dietary adjunct to lower serum cholesterol in vivo.

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Changjian Liu, Qiu Liu, Bo Jiang. Acid and bile tolerance and cholesterol reduction ability of Lactobacillus paraplantarum. [J]. Acta Microbiologica Sinica, 2009, 49(9): 1176-1179

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History
  • Received:March 06,2009
  • Revised:April 27,2009
  • Adopted:
  • Online: March 13,2012
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