Diversity of soil Archaea in Tibetan Mila Mountains

Abstract:

Abstract: [Objective] In order to study the diversity of archaea and ammonia-oxidizing archaea (AOA) of the alp prairie soil in Mila Mountain of Tibet. [Methods] Total microbial DNA was directly extracted from the alp prairie of Mila Mountain. The clone library of 16S rRNA genes and amoA genes were amplified by PCR with universal primer sets. Through 16S rRNA gene and amoA gene cloning library technology to analysis the composition of Archaea and AOA community structure in the alp prairie soil of Mila Mountain. [Results] Phylogenetic analysis revealed archaea in the soil of Mila Mountain including the Crenarchaeota (71.7%) and unclassified_Archaea (28.3%) phyla. All the Crenarchaeota belong to the Thermoprotei. Phylogenetic analysis revealed AOA in the alp prairie soil of Mila Mountain belonged to the kingdom Crenarchaeota. Through DOTUR software analysis, Archaea and AOA species composition from Mila Mountain included 64 OTUs and 75 OTUs. [Conclusion] These findings show prolific archaeal diversity in the alp prairie soil of Mila Mountain, where they may be actively involved in nitrification. In addition, there have some unclassified Archaea in the clone library, it means that they may represent some novel archaeal groups.and what? Meaningless!