Pathogenicity of Hp jhp947 gene to C57BL/6 mice?

Abstract:

Abstract [Objective] To study the effects of Helicobacter pylori jhp947 gene on the pathogenesis of epithelial cells and gene express pattern by animal studies . [Methods] Twenty-seven special pathogen free (SPF) C57BL/6 mice were divided equally into 3 groups, and challenged with Helicobacter pylori J99, Helicobacter pylori△J99-947 and phosphate buffer (PBS) respectively, at a dose of 109 colony formine unit (CFU) at 0, 2, and 4 days. Mice were sacrificed 4 weeks after the last challenge, and went through rapid urease test, culture of Hp, histological examination ,immunofluorescent histochemistry and semiquantitative reverse transcription PCR (RT-PCR) of gastric mucosa. [Results] The result of rapid urease test and culture of Hp indicated that the positive rates in J99 and △J99-947 group were both 100% while 0% in PBS group. The result of histology examination indicated that garstirc mucosa is all normal in PBS group; in J99 group, 33.3% (3/9） had slightly anabrosis, 66.7% (6/9) had seriously anabrosis; in △J99-947 group, 22.2% (2/9) is normal, 77.8% (7/9) had slightly anabrosis. The degree of anabrosis seems to be more severe in J99 than in △J99-947. The result of immunofluorescent histochemistry and semiquantitative RT-PCR of gastric mucosa indicated that the expression level of ets homologous factor, N-myc downstream regulated gene 1, methylthioadenosine phosphorylase is significantly lower in J99 than in △J99-947 group (P<0.05). [Conclusion] The degree of anabrosis seems to be more severe in Hp with jhp947 gene than in Hp without jhp947 gene. In vivo, jhp947 may induce tumorigenesis by inhibiting anti-oncogenes（N-myc downstream regulated gene 1 and methylthioadenosine phosphorylase）.