Reverse genetic system for rabies virus vaccine Evelyn-Rokitnicki-Abelseth strain

Abstract:

Abstract: [Objective] To establish a reverse genetic system of rabies virus for producing safe and efficient rabies vaccine. [Method] By reverse genetic and molecular cloning technique, we established two rabies virus rescue systems including (1) help plasmids expressing N, P and L protein, and (2) CMV/T7 and T7 promoter. [Results] Wild-type rERA-VC was rescued by both systems, and had the same growth kinetics as parental virus. The third generation virus could grow to high titer. [Conclusion] The established reverse genetic system for rescuing wild-type rERA-VC provides the possibility of producing safe and efficient rabies virus vaccines.