Antifungal Activities and Stability of Extracts from Culture Liquid of Hypoxylon perforatum to Sphaeropsis sapinea
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?Supported by the State "Eleventh Five-Year" scientific and technological project(2006BAD08A11105); National Natural Science Foundation(30271083); International cooperation projects in Heilongjiang Province(WC05C01)

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    Abstract:

    Abstract: [Objective] Our research objective is to obtain the active substances from culture liquid of Hypoxylon perforatum with inhibitory effect on Sphaeropsis sapinea growth and germination. [Methods] Water and ester were used for extracting active substances from culture liquid of Hypoxylon perforatum, either by extracting directly from cultural liquid, or extracting under ultrasonic. Growth-inhibiting rate and germination-inhibiting rate were used as the index of antifungal activity. [Results] Five extracts from culture liquid of Hypoxylon perforatum showed antifungal activities to Sphaeropsis sapinea growth and germination. [Conclusion] Ethyl acetate–extract from culture liquid of Hypoxylon perforatum has a highly antifungal activity to Sphaeropsis sapinea, high stability in the natural environment. So it has a highly development of the value and application prospect. The mainly inhibiting active substance is methyl p-methoxy cinnamate, which as medicine intermediate was used in cosmetic to be UV-protection absorbefacient. Diisobutyl phthalate and Dibutyl phthalate are all the common plastic-enhancer for PolyvinylChloride (PVC). It is very important in medicinal industry and chemistry industry to discovering the natural presence of the three compounds.

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Ruiqing Song, Haiyan Gao. Antifungal Activities and Stability of Extracts from Culture Liquid of Hypoxylon perforatum to Sphaeropsis sapinea. [J]. Acta Microbiologica Sinica, 2009, 49(7): 910-917

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  • Received:February 20,2009
  • Revised:April 23,2009
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