Impact of glutathione on the gene expression of exoY and exoS in Pseudomonas aeruginosa

Abstract:

Abstract: [Objective]: To study the impact of GSH (glutathione) on the gene expression of exoY and exoS in Pseudomonas aeruginosa. [Methods]: We treated P. aeruginosa with BSO (buthionine sulfoximine) and DEM (diethylmaleate) to deplete GSH, or construct the P. aeruginosa mutant containing a lacZGm disrupted gshB (glutathione synthetase) gene by homologous recombination technology. The expression of exoY and exoS was determined by measuring light production of the lux-based reporters on pMS402. [Results]: The expression of exoY and exoS decreased in the gshB mutant and P. aeruginosa treated with BSO and DEM. [Conclusion]: GSH in the P. aeruginosa can increase the expression of the genes exoY and exoS. Furthermore, this result provided possibilities to elucidate the molecular mechanisms of pathogenesis and immune response triggered by P. aeruginosa.