Construction of deinococcal bacteria - Escherchia coli shuttle vector and expression of Luciferase gene

Abstract:

Abstract: [0bjective] To express Luciferase gene in Escherchia coli through developed Deinococcal bacteria - E. coli shuttle expression vector. [Methods]The D. bacteria - E. coli shuttle expression vector pZT17 was constructed based on plasmids of pUE30, pGBM5 and pKatCAT. Then pZT17 with lux+ from Photinus pyralis was used to transform into D. grandis and E.coli. The recombinant strains were induced separately. [Results] Based on a small cryptic plasmid from Deinococcus radiopugnans, a shuttle vector between Escherichia coli and deinococcal bacteria was constructed. The plasmid vector could stably aintained in Deinococcus grandis under non-selective conditions. Moreover, it is showed that a luciferase gene was highly expressed both observed in D. grandis and E.coli. [Conclusions]The D. bacteria - E. coli shuttle vector was constructed successfully, the developed shuttle vector makes it possible to induce expression of DNA damage and repair gene from Deinococcus species.