Preparation of the Vaccine with Inactivated Feline Panleukopenia Virus Isolated from Tiger and the Preliminary Application

Abstract:

Abstract: [Objective] To prepare a vaccine with inactivated Feline Panleukopenia Virus (FPV) isolated from Siberian tigers and to evaluate its immunological effect. [Methods] FPV-HLJ, an FPV strain previously isolated from Siberian tiger in our lab was used to inoculate cat kidney cell line F81 with dose 1/10(v/v) using the synchronizing inoculation method. Inoculated F81 cell line was cultured at 37°C and collected when cytopathic effect was up to 75%. Viral suspension was inactivated by using formaldehyde for 24 h and inactive vaccine preapred by adding aluminium hydroxide gel as adjuvant to the suspension. The inactive vaccine was applied to 2- month old kitten tigers of hypodermically after protection effect was proved in 2-month old nonimmunized cats by using the same vaccination procedure. [Results] Antibody level in vaccinated cats revealed an increasing trend that the valence of antibody reached 1:1024~2048 after three times of vaccination. All vaccinated cats survived challenges of virulent FPV virus. The valence of antibody reached 1:1024 in most vaccinated tigers 15 days after the third vaccination. [Conclusion] The results indicated that the inactivated vaccine can produce immunoprotection for tigers.