Probiotic characteristics of Ligilactobacillus salivarius ATCC 11741

Abstract:

[Objective]Certain strains ofLigilactobacillussalivariuspossess excellent probiotic properties and strong potential for application.This study characterized strain ATCC 11741 in terms of the growth characteristics,stress tolerance,and adhesion capacity,aiming to provide a theoretical basis for the utilization of L.salivarius. [Methods]L.salivarius ATCC 11741’s accuracy was verified through morphological observations and 16S rRNA gene sequencing;its growth characteristics were investigated by tracking growth and acid production curves;additionally,a range of tolerance tests,including those with acid,alkali,bile salts,hyperosmolarity and temperature,were conducted to evaluate its tolerance;lastly,self-aggregation and hydrophobicity experiments were used to indirectly determine its adhesion.[Results] The growth curve of L.salivarius ATCC 11741 was formed like a “S”.The strain reached its highest acid production between 2–7 h,stabilizing at pH 4.3 after 14 h.After being cultured in the MRS medium at pH 2.0 for 4 h,the strain had a survival rate of 50.48% and grew well in the pH 4.0–11.0 range.Following 2 h of incubation in the MRS medium with 0.10% bile salt,the strain’s survival rate was 94.440 0%.In the MRS medium with 6% NaCl,the strain continued to proliferate.The growth of strain ATCC 11741 was significantly affected by temperature,being promoted at 30–42℃ and inhibited at 20℃ and 50℃.At the 5 h mark,the strain’s hydrophobicity (measured by the hydrocarbon compound adhesion method) was 44.5% and its self-aggregation rate was 41.4%.[Conclusion] L.salivarius ATCC 11741 is a strain with fast growth,strong tolerance to acids,alkali,and salts,moderate tolerance to bile salt,a wide range of suitable temperatures,and strong adhesion capacity.