Progress in experimental techniques for the discovery and target determination of pathogen sRNAs targeting hosts

Abstract:

People are exposed to environments containing various pathogens, which have multiple interactions with human cells or tissues. Pathogens can survive in the host environment by regulating pathogenic conditions such as virulence and invasiveness. At the same time, host cells resist the invasion of pathogens by mobilizing their own immune system. However, researchers mainly focus on the physiological functions of sRNAs in pathogens and have gained limited knowledge about the interactions between pathogens and hosts. How to use highly sensitive and high-resolution methods to study the interactions between pathogens and hosts have become a major challenge in the current research. By reviewing relevant studies, we summarize the commonly used techniques and experimental processes for studying the interactions between pathogens and hosts, aiming to improve the understanding about the mechanisms and principles of these experimental techniques and provide technical references for the research on interactions between pathogen sRNAs and host targets.