Bacterial diversity in human colostrum samples from different regions

Abstract:

The microorganisms in colostrum have a variety of beneficial effects on the growth and development of infants. [Objective] We analyzed several factors affecting the flora composition of colostrum, aiming to provide a new idea for the follow-up study of human colostrum flora. [Methods] We used PacBio SMRT sequencing platform to complete the bacterial 16S rRNA gene sequencing of 37 colostrum samples collected from Enshi, Hubei Province. We then used the sequence data and the published data of 62 human colostrum samples from other regions in the public database to explore the composition and structure of bacterial flora in the human colostrum samples from different regions. [Results] There were 345 genera and 937 species in 99 colostrum samples. Lactococcus lactis, Ralstonia insidiosa, and Gemella haemolysans were the dominant species in human colostrum. The principal coordinate analysis (PCoA) based on jaccard distance showed a significant separation trend of flora in human colostrum samples between different regions. The R² of the factors affecting the composition of colostrum flora followed the trend of region>infant feeding modes>maternal health during pregnancy>mode of delivery>parity. [Conclusion] Region was the main factor affecting the flora composition of human colostrum.