Abstract:The metabolic diversity of anaerobic ammonia-oxidizing bacteria (AnAOB) allows them to widely distribute in different natural ecosystems such as oceans, wetlands, and land, which are even detected in some extremely hot and cold environments. In this review, the discovery, distribution, and denitrification contribution of anaerobic ammonia-oxidizing bacteria in different ecosystems were reviewed and summarized, and the main environmental factors affecting their distribution were analyzed as well. This review may help us understand the real role and function of anaerobic ammonia-oxidizing bacteria better in the global nitrogen cycle. Based on the anaerobic ammonia oxidation (anammox) process, this paper explored novel biological nitrogen removal processes that could cooperate with it, to provide an ecological basis and new ideas for the promotion and development of the processes, thereby realizing the technological transformation of denitrification process.