Abstract:[Objective] Pseudomonas boreopolis GO2 synthesizes bioflocculant with lignocellulosic biomass. In this study, the whole genome sequencing and comparative genome analysis of GO2 were performed to explore its genome characteristics and analyze genes related to lignocellulose degradation and polysaccharide bioflocculant synthesis. [Methods] Illumina NovaSeq was used for genome sequencing, and SMRT and other software for genome assembly, phylogenetic analysis, and gene prediction and annotation. Moreover, the genome was respectively compared with that of four similar model strains. [Results] The genome of GO2 was 4 498 896 bp, with guanine-cytosine (GC) content of 69.5% and 3 906 coding genes. It showed the highest 16S rRNA gene similarity (99.93%), DNA-DNA hybridization (DDH) (88.00%), and average nucleotide identity (ANI) (98.36%) to P. boreopolis JCM 13306. Therefore, this strain was named as P. boreopolis GO2. GO2 shared 2 348 core orthologous genes with the four model strains, and these genes were mainly involved in the pathways of carbohydrate metabolism, amino acid metabolism, and energy metabolism. The 307 specific genes of GO2 were mainly involved in the pathways of transcription, replication, repair, and cell wall/membrane biogenesis. GO2 contained 226 carbohydrate-active enzymes (CAZymes) genes, accounting for 5.79% of the total number of genes. Among the CAZymes genes, 82 belonged to the glycoside hydrolases (GHs) family which was related to plant cell wall degradation and 3 clusters of polysaccharide synthesis genes were formed by glycosyltransferases (GTs) family genes. [Conclusion] Based on the comparative analysis of genome sequences, GO2 is named Pseudomonas boreopolis GO2, and its genome contains rich plant cell wall degrading enzyme genes and three polysaccharide synthesis gene clusters, which may play an important role in the synthesis of bioflocculants by with lignocellulosic biomass in GO2.
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